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#Antibody sequence analysis full
#Antibody sequence analysis plus

Identify variable domains and leader sequences using 5’RACE, clone and express the identified antibody in 10 ml culture for testing.

#Antibody sequence analysis plus

Antibody Sequencing plus Test Expression.

Delivery Time: 3 weeks – Price: starting from $1450. Identify variable domains and leader sequences using 5’RACE reactions this is the standard way in the field to assert the exact sequence of the variable domains of your antibody and important step to take when translating an antibody into clinic. Delivery Time: 2 weeks – Price: starting from $950. Mutations, normally silent, may be introduced at junctions not all species available, please inquire. Identify variable domains using primer sets, perfect for routine work during antibody generation and re-cloning as scFv. Sequencing analysis up to 5x coverage or 10 clones per chain, whichever come first. This is a “ No Success – No Fee” service you pay on delivery of your antibody sequence.Īmplification of VH and VL domains including leader sequence and partial constant regions CH1 and CL.Ĭolony PCR and sequencing of clones with proper insert size. Hybridomas are prone to contain multiple chains as a result of cellular fusions involving multiple B-cells (quadromas), non-productive rearrangements generated during B-cell maturation and pseudogenes we will conduct a thorough and extended analysis of multiple clones to confirm the identity of the relevant full-length, in-frame sequences and eliminate aberrant endogenous myeloma light chains. We can sequence from a variety of species including mouse, rat, rabbit and hamster hybridomas or human EBV-immortalized B-cell lines and isotypes including IgM & IgA.

#Antibody sequence analysis full

A full length sequencing service including complete leader sequence using 5’-RACE and sequence of constant domains (full antibody) is also available upon request. Both light chain and heavy chain variable domain cDNAs will be amplified using either a combination of leader primers or state-of-the-art rapid amplification of DNA ends (5’-RACE). Stable cell line quality control & Traceability.Ī ntibody Design Labs offers a fast, reliable and competitively-priced sequencing service to clone your antibody cell line. Patent applications & Intellectual property. Knowing the sequence of your antibodies will protect you from the loss of your valuable clones it is one of the first steps to get protection of your antibodies through patent applications and it is an important quality control to maintain consistent cell lines for batch production. Please contact us if you are interested in sequencing your antibody of interest and our experts are willing to help and discuss your needs in detail.G etting accurate sequence of the variable domains of your monoclonal antibodies will open up a realm of possibilities for downstream antibody engineering from in vitro recombinant expression to affinity maturation and humanization. Leucine and isoleucine are assigned based on preferences of enzyme cleavage sites and frequencies in our database.īesides the primary amino acid sequence of heavy and light chains of the antibody, Creative Proteomics is also able to characterize post-translational modifications such as glycosylation and others according to customers’ requests. We only need a few hundred micrograms of an antibody with a minimum purity of 95% for de novo antibody sequencing. Eventually, a comprehensive result report including raw MS data is provided to our customers. Lastly, our senior specialists perform data analysis by using the most advanced computational algorithms, which efficiently derive the amino acid sequence of digested peptides and assemble overlapped peptides. Creative Proteomics uses hybrid quadrupole-Orbitrap mass spectrometers for data acquisition, which generates data with superior quality: high resolution and accuracy.

Then the digested peptides are analyzed by LC-MS/MS. If required, the heavy and light chains of the antibody can be separated prior to enzymatic digestion. In a typical workflow, the antibody is digested by using a panel of different enzymes (either one at a time or a combination of a few) including the most commonly used one: trypsin and several others such as Glu-C, Asp-N, chymotrypsin and etc. Equipped with state-of-the-art technologies such as high-performance liquid chromatograph coupled to tandem mass spectrometry (LC-MS/MS) and advanced computational algorithms, Creative Proteomics can offer accurate and fast antibody de novo sequencing service customized to your needs.ĭe novo sequencing of antibodies refers to the analytical process of deriving the amino acid sequence of an antibody and discovering any associated post-translational modification without knowing DNA or protein sequence beforehand.